Cloned (Comment) | Organism |
---|---|
expressed in the Escherichia coli BL21 (DE3) | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the enzyme in complex with the inhibitor O-isobutenyl oxalylhydroxamate and with the cofactor NAD+ at 2.1 A resolution | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O-isobutenyl oxalylhydroxamate | competitive inhibitor, binds the active site by adopting the same conformation of (2R,3S)-3-isopropylmalate, and to induce an unreported repositioning of the side chain of the amino acids that participate in the coordination of the ligand | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Haemophilus influenzae | the enzyme belongs to the leucine biosynthetic pathway | 4-methyl-2-oxopentanoate + CO2 + NADH + H+ | - |
ir | |
(2R,3S)-3-isopropylmalate + NAD+ | Haemophilus influenzae ATCC 51907 | the enzyme belongs to the leucine biosynthetic pathway | 4-methyl-2-oxopentanoate + CO2 + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P43860 | - |
- |
Haemophilus influenzae ATCC 51907 | P43860 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Haemophilus influenzae | 4-methyl-2-oxopentanoate + CO2 + NADH + H+ | - |
ir | |
(2R,3S)-3-isopropylmalate + NAD+ | the enzyme belongs to the leucine biosynthetic pathway | Haemophilus influenzae | 4-methyl-2-oxopentanoate + CO2 + NADH + H+ | - |
ir | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Haemophilus influenzae ATCC 51907 | 4-methyl-2-oxopentanoate + CO2 + NADH + H+ | - |
ir | |
(2R,3S)-3-isopropylmalate + NAD+ | the enzyme belongs to the leucine biosynthetic pathway | Haemophilus influenzae ATCC 51907 | 4-methyl-2-oxopentanoate + CO2 + NADH + H+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
leuB | - |
Haemophilus influenzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Haemophilus influenzae |
General Information | Comment | Organism |
---|---|---|
drug target | since leucine is an essential amino acid for humans, and it is also vital for the growth of many pathogenic bacteria, the enzymes belonging to this pathway can be considered as potential target sites for designing of a new class of antibacterial agents | Haemophilus influenzae |
metabolism | the enzyme belongs to the leucine biosynthetic pathway | Haemophilus influenzae |